risperdal 4 mg side effects side effects of nexium 40 nom generique plavix side effects of avalide 300-125 mg seroflo 250 inhaler buy 102 años plus con ginseng micardis 40 precio amaryl 5500 compra casa a cipro que es altace 5 mg y para que sirve ciprofloxacine generique de quoi 240 mg xenical trental 100 mg 5 ampul norfloxacin 400 mg tinidazole 600 mg shatavari comprar zetia precio mexico 15 mg prednisolone side effects saw palmetto 500 mg ga200 gasex motilium 10 mg efectos secundarios tricor efectos secundarios cuales son los efectos secundarios del coumadin pastillas dostinex ginseng pastillas does crestor come in 80 mg irwin naturals hoodia action vytorin 1020 comprar exelon yasmin anticonceptivas precio bijwerkingen paroxetine 10 mg etodolac er 500 natural aspirin plants precio flagyl perros kegunaan amitriptyline 25 mg proventil 200 aciclovir pastillas herpes bucal differin 03 efectos celadrin 50 ml relafen 250 mg cytotec versus natural miscarriage mobic 200 mg natural remedy for valtrex 10 day hoodia diet viagra super active comprar inderal precio acai 1000 mg propiedades zofran 4 mg tabletas 325 mg aspirin vs coumadin efectos secundarios de geriforte benzac 30 ratio metformin 500mg how much is amoxicillin 500mg lexapro efectos al dejar de tomar tab bupron xl 150 use of zenegra 100 efectos secundarios de cephalexin 500mg cozaar plus efectos secundarios vantin 500 mg atrovent kapsel 40 mikrog mac floxin 750 vytorin 1040 generico ratio metformin 500mg precio januvia 25 mg comprar ventolin strattera 70 mg retin a precio costa rica generic cardizem cd 360 mg lamictal dispersable efectos secundarios cialis 10 hytrin 1mg tablet precio viramune zovirax pomada oftalmica efectos secundarios etodolac er 600 mg precio alquiler salon de actos 20 mg claritin per day adaalat 7 march 2015 dailymotion lamictal 400 mg bipolar calcium carbonate 500 mg tab Breakthrough Method of Stem Cell Expansion

Breakthrough Method of Stem Cell Expansion


Yupo Ma, MD, PhD, Professor of Pathology Stony Brook

Breakthrough Method of Stem Cell Expansion

Released:6/6/2011 1:00 PM EDT
Source:Stony Brook University Medical Center

Newswise — STONY BROOK, N.Y., June 6, 2011 – Researchers in the Department of Pathology at Stony Brook University School of Medicine have discovered a laboratory method to expand adult hematopoietic stem cells (HSCs) using the SALL4 gene. Professor Yupo Ma, M.D., Ph.D., Lead Author, and colleagues used this method to produce a more than 10,000-fold increase in HSCs derived from normal human bone marrow. Their findings define a new mechanism of stem cell self-renewal, providing a means to produce large numbers of HSCs that could be used to treat hematological malignancies and other blood disorders. Their results are reported in the early online edition of Blood.

HSCs are rare cells capable of differentiating into all blood cell lineages. These cells are used in clinical procedures to treat various blood diseases, including leukemia and lymphoma, and are central to bone marrow transplantation. Only one in three patients in need of bone marrow transplantation are matched with a suitable donor, and thus many patients die before finding a match. The Stony Brook research team’s discovery of using SALL4, which produces a protein that stimulates HSCs to grow, may be a critical first step to finding a treatment based on the expansion of HSCs that could become one alternative to finding a matched donor.

“Investigators have been trying to expand human HSCs for 30 years, but so far there have been only small incremental advances without clinically meaningful results,” says Dr. Ma. “We believe our findings are so different from others that this method could open the door to a process that expands HSCs and be used clinically.”

The research team, spearheaded by co-author Jerell R. Aguila, Ph.D., devised a way to transfer a stem cell gene (SALL4) into human bone marrow HSCs (CD34+ and CD38-) using a viral delivery system. Once the cells started producing the SALL4 protein, they were exposed to chemical agents known as cytokines, in order to mimic the environment of normal bone marrow.

By using SALL4 transduction methods, Dr. Ma’s team of investigators was able to increase the number of HSCs by a factor of 10,000-to-15,000 fold. Subsequently, the team demonstrated that these cells could replace and expand into bone marrow stem cells. The team then successfully transplanted cells from primary recipients and transplant them into a secondary recipient, and from the secondary recipient into a tertiary recipient.

Data presented in the Blood article, titled “SALL 4 is a robust stimulator for the expansion of hematopoietic stem cells,” support the conclusion that this new method is far superior to existing approaches. Using the SALL4 transduction methodology, they found HSC expansion consistently resulted in 10,000-to-15,000 fold increases in the cells. Previous methods to expand HSCs have had limited success, with a 160-fold increase achieved by only one of the methods.

Furthermore, no adverse effects were detected in animal models that were monitored for more than 12 months. Dr. Ma’s team was also able to eliminate the need for viral delivery of SALL4 by generating a novel recombinant (TAT-SALL4) protein, making it possible to translate their work into the clinical setting.

“The achievement of Dr. Ma and his research team is an important milestone along the road to developing new methods to treat leukemia and lymphoma and may someday be applied to create stem cells from other tissues, including heart, pancreas, and muscle,” says Kenneth Shroyer, M.D., Ph.D., Chair of the Department of Pathology at Stony Brook University School of Medicine.

“Ultimately, this method could accelerate the use of stem cell therapies for cancer, as well as a broad range of other diseases,” Dr. Shroyer adds.

The study co-authors include: Wenbin Liao, Ph.D., Nabil Hagag, Ph.D., and Lisa Senzel, M.D., Ph.D., of the Department of Pathology at Stony Brook; Cecilia Avila, M.D., of the Department of Obstetrics & Gynecology at Stony Brook, and Jianchang Yang, M.D., Ph.D., of the Nevada Cancer Institute.

The Blood and Marrow Stem Cell Laboratory at Stony Brook University Medical Center provided the cells used in the research, which was supported, in part, by several grants from the National Institutes of Health (NIH).

About Stony Brook University School of Medicine:
Established in 1971, the Stony Brook University School of Medicine includes 25 academic departments centered on education, training, and advancing scientific research. The primary mission of the School is to educate caring and skilled physicians well-prepared to enter graduate and specialty training programs. The school’s graduate and specialty training programs are designed to educate medical specialists and investigators in the biomedical and clinical sciences to be well-prepared to advance the frontiers of research, clinical practice and education.





  1. Pingback: cool caravans

  2. Pingback: cheap guest beds

  3. Pingback: triactol

  4. Pingback: young women's clothing

  5. Pingback: tallOutdoorBarStools

Welcome to Investor Stemcell! New to the site? Take a minute and Sign Up Today!